Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium Size:500g 45 g/l Casein Peptone 5000

45 g/l Casein Peptone 5000 g/l NH4Cl 26

0  M  60

Tryptone 12 Yeast Extract 24 Agar 15 Final pH 7

It is a structural analog to MES and it’s chemical structure contains a morpholine ring

Used as a selective marker for the transformation of plasmids encoding for tetracycline resistance (Tetr)

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium Size:500g 45 g/l Casein Peptone 5000Magnesium sulphate, MgSO4. 7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 2 gram powdered medium in 1 litre distilled water Store