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reverse transcription reactions
releasing 5'-phosphorylated mononucleotides or oligonucleotides
Integrated genomic DNA removal and cDNA synthesis in one workflow
The enzyme lacks RNase H activity and has high tolerance to common reverse transcriptase inhibitors
- Incorporation of modified nucleotides (such as biotin
AugeGreen qPCR Dye, 20X in Water Cat. No./Spec.:P6031-B/5ml reverse transcription reactionsDescription AugeGreen is a DNA binding dye used for real time quantitative PCR (qPCR). It has similar spectral characteristics to SYBR Green I but offers significant advantages such as low PCR inhibition, high stability, and high safety, making it an ideal substitute for SYBR Green I, especially in qPCR reactions requiring high resolution melting curve analysis. Product Parameters Absorption wavelength (abs) for unbound DNA: 471 nm Emission wavelength
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