Luciferase-Expressing Canine Primary Colonic Fibroblasts Cell Culture Reagents 40 cycles of 95°C 0:15

40 cycles of 95°C 0:15 and 60°C 1:00 with signal read at 60°C

Defects in this gene cause immunodeficiency

GFP-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colon tissues of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium

Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue or GP6 antigen-treated (Neutralization experiment) using P111730(GP6 Antibody) at dilution 1/50

followed by secondary steps with RNase A to remove RNA

Luciferase-Expressing Canine Primary Colonic Fibroblasts Cell Culture Reagents 40 cycles of 95°C 0:15Description Luciferase Expressing Canine Primary Colonic Fibroblasts from Cell Biologics are isolated from the colon tissue of beagle dog and grown in gelatin pre coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2 3 are harvested from flasks and cryo preserved in vials. Each vial contains 0. 5x106 cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for