Small RNA Library Prep Kit for Illumina Methylation Bisulfite The SYBR Green I dye

The SYBR Green I dye binds to double-stranded DNA (dsDNA)

and Bio-Rad

but it is inactive on single-stranded nucleic acids

Amplification of complex template

but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→5´ exonuclease activity

Small RNA Library Prep Kit for Illumina Methylation Bisulfite The SYBR Green I dyeThe Small RNA Library Prep Kit for Illumina is designed to construct small RNA sequencing libraries for Illumina platforms. It ligates universal adaptors to the 3' and 5' ends of small RNAs, followed by reverse transcription, PCR amplification, and purification via PAGE gel or magnetic beads. It supports total RNA inputs from 10 ng to 1 g, as well as purified serum, plasma, and exosome RNA (1 ng). Strict quality control ensures consistent library